Using Ovulation Tests to Predict Fertility
Luteinizing Hormone, the LH Surge, and Ovulation
At the beginning of the menstrual cycle, the body begins to produce follicle stimulating hormone (FSH). FSH facilitates the formation of a follicle on one of the ovaries. The follicle contains and nurtures the egg. When a follicle has adequately matured, a surge of Luteinizing Hormone (LH) causes the follicle to burst and release the egg into the fallopian tube - the moment of ovulation.
Throughout the menstrual cycle, a small amount of LH is produced - but during the middle of the cycle LH briefly and dramatically increases. Elevated quantities of luteinizing hormone facilitate ovulation - and OPKs detect this LH surge through anti-LH antibodies contained in the sensitive testing membrane of the test.
The LH surge is, alas, very brief - and in order to detect the LH surge, a woman needs to test at the right time of the month - and the right time of day. As LH is produced by the body in the morning, mid-afternoon is considered the ideal time to test.
Once the LH surge has been detected, successful fertilization is most likely to take place one to three days following the LH surge - with peak fertility at 36 hours post-LH surge. Since this ovulation "window" only opens once per month (and the unfertilized egg has a short 24-hour life-span) predicting ovulation accurately is very helpful when trying to become pregnant. Below, you will find information on getting the most out of your ovulation tests (and visit our Ovulation Test FAQ for more testing tips).
Advanced Testing Tips and Facts: Answering Common Questions about OPKs
Using OPKs and Interpreting Ovulation Test Results
Ovulation tests come in two formats: test strips and midstream tests. To use the ovulation test strip, you fill a container with urine and hold the test strip in the container for several seconds. With midstream tests, you hold the test in your stream of urine. Both test formats are equally reliable. Always read ovulation test kit instructions carefully.
With most home ovulation testing products, the "reaction time" of the test is five minutes. At five minutes, you can interpret the tests. All tests have a "control" color band (or color line) that indicates whether the test is working or not. Also, the control band provides a color/intensity baseline by which to interpret the "test" band results. The "test" color band indicates a positive or negative result (please refer to the graphic below).
A positive result (indicating an LH Surge) is indicated by a test band that is of equal or greater intensity (equal or darker) than the control band. A negative result for the LH Surge is indicated when the test band is of lesser intensity (lighter) than the control band or cannot be seen. A negative result means the LH level of the urine sample is at or near its normal level and that the LH surge is not in progress. Remember, there is always some quantity of LH in your system, so a light color band in the test region is NOT an indicator of a positive result.


How do I Determine when to Begin Testing?
To determine when to start testing, you must first do a little calculating. Use the Cycle Chart below to figure out when to begin testing for your LH surge.
First, determine the average length of your menstrual cycle. The length of the menstrual cycle is the number of days from the first day of menstrual bleeding to the day before bleeding begins on the next period. Determine the usual length of the menstrual cycle over the last few months. Then, refer to the Cycle Chart to determine on which day of the menstrual cycle to begin testing.
| Your Cycle Length |
Day to Begin Testing |
| 21 days | Day 5 |
| 22 days | Day 6 |
| 23 days | Day 7 |
| 24 days | Day 8 |
| 25 days | Day 9 |
| 26 days | Day 10 |
| 27 days | Day 11 |
| 28 days | Day 12 |
| 29 days | Day 13 |
| 30 days | Day 14 |
| 31 days | Day 15 |
| 32 days | Day 16 |
| 33 days | Day 17 |
| 34 days | Day 18 |
| 35 days | Day 19 |
| 36 days | Day 20 |
| 37 days | Day 21 |
| 38 days | Day 22 |
| 39 days | Day 23 |
Read More About:
> Ovulation Test FAQ: Testing Tips and Tricks
> Saliva Based Ovulation Predictors
> Ovulation FAQ: Frequently Asked Questions About Ovulation
> Pregnancy Testing
> OvaCue Product Review
Comments
I USED MY OPK SATURDAY MORNING @ 6AM AND RECEVIED A POSITIVE. LATER THAT DAY ABOUT 1PM I STARTED HAVING VERY SEVER OVULATION PAINS WHICH I GET EVERY MTH. IT'S AS IF THE EGG BURST OUT AT THAT MOMENT I GUESS. ANYWAY I HAD SEX ABOUT 1AM SATURDAY MORNING. IM WONDERING IF SATURDAY WAS MY ACTUAL DAY I OVULATED.
I've been having positive OPK results for 6 days now. My temperature started to rise yesterday, when I got my first negative (or close to negative: the two lines seem to be the same color, but the control line is slightly thicker...). Does anyone know what it means? I never had positive results before, even when I was on clomid...
Is it normal to have a positive test results for 5 days on an ovulation test?
Ive been taking Clomid for the last 5 days, today is Day 7.
When should i start testing? my last cycle was 27 days and the one before that, 35 days.
They also turn positive if you're pregnant. Or the test could be faulty, or you misinterpreted the results.
Um...I have been charting my BBT and started on 7-29-09. I took my temp this morning it dropped to 96.8 and so I used an OPK...said positive....I am on cycle day 6...what is the deal...and my cycle is 33 days long.
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